Localization of mouse satellite DNA on chromosomes of experimentally induced glioblastomas; non-centromeric lable in one glioblastoma producing C-type particles
✍ Scribed by Laura Manuelidis; Elias E. Manuelidis
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- French
- Weight
- 1014 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
The chromosomal localization of satellite DNA in two tissue culture lines derived from malignant mouse CNS tumors was investigated by in situ hybridization of ^3^H single‐stranded satellite DNA purified by isopynic centrifugation in alkaline C~s~Cl. Both tumors were glioblastomas originally induced by a methyl‐cholanthrene implantation into the cerebrum of C~3~H mice; both displayed aneuploid chromosomal constitutions. One of these glioblastomas (TC 541) revealed labelling only of centromeric portions of the chromosomes even in cells containing >200 chromosomes and thus it had a pattern of satellite distribution comparable to that of normal cells. The other glioblastoma (TC 509), that produced C‐type particles and had a decrease in satellite DNA, displayed interstitial and telomeric label in some chromosomes in addition to labelling of the centromeres. “Hoechst 33258” fluorescence showed some interstitial and telomeric bright bands as well as centromeric bright regions, thought to be consistent with in situ studies. The localization of satellite DNA to the chromosome arms and its possible relation to C‐type virus is discussed.