The motor pools of the individual abdominal muscles of the cat were localized in studies by using either intramuscular injections of horseradish peroxidase (HRP) to retrogradely label abdominal motoneurons or electrical microstimulation of the ventral horn at different segmental levels to produce localized twitches of the abdominal muscles. The segmental distribution of each motor pool was as follows: rectus abdominis, T4-L3; external oblique, T6-L3; transverse abdominis, T9-L3; and internal oblique, T13-L3. The differences in the rostral extents of the individual motor pools reflect the greater rostral extents of the different muscles (rectus abdominis > external oblique > transverse abdominis > internal oblique). Labeled motoneurons were also found at other segmental levels; however, it was concluded that this labeling occurred because of spread of HRP from the injected muscle since localized abdominal muscle twitches could not be produced by electrical stimulation in these regions. In addition, control experiments showed that HRP can spread from the injected muscle and identified the sources of some of this spurious labeling. Motoneurons labeled after injections into the four abdominal muscles overlapped extensively on transverse sections of the spinal cord; however, rectus abdominis motoneurons were located more medially than the others from about T11 to L3. Soma diameters ranged between 12 and 41 pm (average 24-26 pm per cat). In summary, this study has provided a systematic description of the innervation of the individual abdominal muscles of the cat.