Localization of mineral ash in the organelles of trichonympha, a hypermastigote flagellate from zootermopsis angusticollis
✍ Scribed by R. F. Maclennan; H. K. Murer
- Publisher
- John Wiley and Sons
- Year
- 1934
- Tongue
- English
- Weight
- 591 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0362-2525
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✦ Synopsis
Abstract
The three species of Trichonympha found in Zootermopsis angusticollis were studied by micro‐incineration, by control sections, and by Macallum's methods for iron and calcium. The mineral ash is concentrated in the neuromotor system, chromatin, nuclear membrane, the posterior end where ingestion takes place, and in cytoplasmic granules. Micro‐incineration and Macallum's Prussian blue method reveal iron in the chromatin alone; Macallum's haematoxylin gives a positive test for iron in the chromatin, rostrum, neuromotor fibrils, etc. It probably is not as specific as the other methods. The two staining methods give negative tests for iron except after acid hydrolysis. Calcium is present in the chromatin, neuromotor system, posterior region of the body, and in many endoplasmic granules. The localization of calcium is correlated with the localization of normally gelated regions.