✦ LIBER ✦
Localization of membrane-bound and soluble antigens using peroxidase-labeled antibodies and preembedding immunoelectron microscopy
✍ Scribed by Osamura, R. Y. ;Watanabe, K.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1985
- Tongue
- English
- Weight
- 781 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0741-0581
No coin nor oath required. For personal study only.
✦ Synopsis
The immunoelectron microscopy preembedding method using peroxidase-labeled antibodies has enabled us to localize various bioactive substances in subcellular organelles, i.e., nucleus, cytosol, perinuclear space, rough endoplasmic reticulum, Golgi complex, secretory granules, mitochondria, etc. In this text, detailed procedures and some key points for the appropriate staining are described. The applications of this technique to localize substances in various organelles and to observe ultrastructural changes of intracellular hormones are-also discussed.