Localization of a gene for human α-galactosidase B (=N-Acetyl-α-D-Galactosaminidase) on chromosome 22

✍ Scribed by P. G. Groot; A. Westerveld; P. Meera Khan; J. M. Tager

• Publisher: Springer
• Year: 1978
• Tongue: English
• Weight: 418 KB
• Volume: 44
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00394295

✦ Synopsis

The localization of the structural gene for human alpha-galactosidase B (= N-acetyl-alpha-galactosaminidase) was investigated by means of man-Chinese hamster and man-mouse somatic cell hybrids. The hybrid clones were analyzed for chromosomes and for a large number of known enzyme markers. The lysates of the hybrid cells were treated with Sepharose-coupled antihuman alpha-galactosidase B and the activity of the adsorbed enzyme was measured on the Sepharose beads as N-acetyl-alpha-galactosaminidase. The results show that the structural gene for human alpha-galactosidase B is situated on chromosome 22, and that there is no structural relationship between human alpha-galactosidase A and human alpha-galactosidase B.