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Localization of 4S RNA genes on the chloroplast genome ofChlamydomonas reinhardii

✍ Scribed by Pia Malnoë; Jean-David Rochaix

Publisher
Springer
Year
1978
Tongue
English
Weight
594 KB
Volume
166
Category
Article
ISSN
1617-4615

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✦ Synopsis

The genes coding for 4S RNA have been localized on the physical map of the chloroplast genome of Chlamydomonas reinhardii by hybridizing 32P-labelled 4S RNA to EcoRI, BamHI, Bg1II and Hind III chloroplast DNA digests and to hybrid plasmids containing EcoRI and Bam HI chloroplast DNA fragments. At least 10 EcoRI and 7 Bam HI fragments carry sequences coding for 4S RNA. These genes are interspersed throughout the genome. The spacer between the 16S and 23S ribosomal RNA genes, which is repeated twice per chloroplast DNA molecule, codes for at least one 4S RNA, shown to be transcribed from the same strand as the ribosomal RNAs.

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Physical mapping of 4S RNA genes on chlo
Physical mapping of 4S RNA genes on chloroplast DNA ofSpirodela oligorhiza
✍ Gert S. P. Groot; Nel Harten-Loosbroek 📂 Article 📅 1981 🏛 Springer-Verlag 🌐 English ⚖ 324 KB

We have determined the position of Spirodela oligorhiza chloroplast 4S RNA genes on the restriction fragment map of cp DNA, using purified in vitro[(32)P]-labeled 4S RNA. The overall organization of these genes is very similar to the organization of tRNAs on spinach cp DNA (Driesel et al. 1979).