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Localization of 25-hydroxyvitamin D3-lα-hydroxylase activity in the mammalian kidney

✍ Scribed by Takashi Akiba; Hitoshi Endou; Chizuko Koseki; Fuminori Sakai; Noboru Horiuchi; Tatsuo Suda

Book ID
119406855
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
315 KB
Volume
94
Category
Article
ISSN
0006-291X

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Until recently measurement of 25-0H-D r Ia-hydroxylase activity in mammalian kidney has not been possible due to the presence of a protein which inhibits the enzyme by reducing available substrate. However, utilization of sufficient unlabeled 25-0H-D 3 (80 nmol/ml renal homogenate) to overcome the e