✍ Scribed by K. G. Haglid; H. Hydén; P. W. Lange
No coin nor oath required. For personal study only.
The 14‐3‐2 acidic protein specific for nerve tissue has been localized to bands 4 and 5 counted from the anodal front band in a polyacrylamide‐gel eletrophoretic pattern. The material consisted of cut‐out layers of pyramidal nerve cells of the CA3 hippocampal region of the rat. Extracted protein samples were subjected to immunoprecipitation, Coons' double‐layer immunofluorescence, microcompletment fixation, and a localization experiment using microdensitometry on a 400 μΦ gel electrophoresis pattern with a without extra added 14‐3‐2 protein. The protein fractions 4 and 5, now shown to contain 14‐3‐2 protein, have recently been found to incorporate increased precursors at training as compared to controls.
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It has been demonstrated that 14‐3‐3 proteins are present in the nuclei of __Arabidopsis thaliana__ and __Zea mays__ cells using laser scanning confocal microscopy and immunocytochemistry with monoclonal antibodies against plant 14‐3‐3 proteins. Confirmation of nuclear localization provides insight
The GF14 family of proteins in Arabidopsis thaliana consists of a homologous group of polypeptides ranging in size from approximately 27 kDa to approximately 32 kDa. As a group, GF14 proteins are also homologous to a family of mammalian proteins most commonly referred to as 14-3-3 proteins. Several
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