Localization and release mechanism of cellulases inTrichoderma reeseiQM 9414

✍ Scribed by Meera Nanda; V. S. Bisaria; T. K. Ghose

• Publisher: Springer Netherlands
• Year: 1982
• Tongue: English
• Weight: 272 KB
• Volume: 4
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00141976

✦ Synopsis

A significant increase in the extracellular yield of 8-glucosidase was observed when Trichoderma reesei QH 9414 was cultivated on a cellulose medium containing chitin. tte asurement of enzyme activities in the various fractions of the mycelium revealed that endoglucanase was truly extracellular while 8-glucosidase was cell wall bound. Treatment of Trichoderma mycelium with cell wall degrading enzymes (produced from Trichoderma) led to a release of B-glucosidase from the mycelium.

Apparently chitin, in the presence of cellulose, induces the synthesis of chitinase and other cell wall lytic enzymes which promote release of the intramural 8-glucosidase into the medium.