Localization and nucleotide sequence of the bom region of Clo DF13

✍ Scribed by Snijders, Alies ;van Putten, Arnold J. ;Veltkamp, Eduard ;Nijkamp, H. John J.

• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 841 KB
• Volume: 192
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00392189

✦ Synopsis

Mobilization of the non-conjugative plasmid Clo DF13 requires both gene products of a conjugative plasmid and Clo DFI3 encoded proteins as well as a cis-acting Clo DF13 DNA region, termed bom (basis of mobility). The born region was located within a 264 bp fragment around the unique HpaI site. Comparison with the corresponding ColEI and pBR322 sequences showed similarities with respect to the secondary structure. With respect to a possible relationship between the origin of vegetative replication (oriV) and the origin of transfer (oriT), we found that neither distance nor orientation of the Clo DF13 born region with respect to oriV had any significant influence on the mobilization frequency. Surprisingly, after cloning of the 264 bp HpaII fragment in a bom-vector, restoration of the born + phenotype was only observed in one orientation. From these observations and from the sequence analysis of the born region we suggest that transcription into this cloned bom fragment is essential for effective mobilization of the plasmid.