Local anesthetics measured by lipophilic β-cyclodextrin-based ion-selective electrodes

✍ Scribed by Ritu Kataky; Simon Palmer

• Book ID: 102833826
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 635 KB
• Volume: 8
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.1140080616

✦ Synopsis

2,3,6-Trioctyl

and

2,6

-didodecyl-~-cyclodextrins have been incorporated in ion-selective electrodes as neutral ionophores for the detection of the local anesthetics procaine, prilocaine, lidocaine and bupivacaine hydrochlorides. Nernstian response and excellent limits of detection were observed (-log [C] 25.0). Interference from 'serum' levels of sodium, potassium and calcium (1 50.0 mmol dm-3 Na+, 1.26 mmol dm-3 Ca2+ 4.30mm0ldrn-~ K+ ), was negligible (-log K r ' ) (overall) 24.0). The major organic interferents were molecules of similar size and structure which caused reduced slope and drift in baseline potentials at 10 mmol dm-3 levels of concentrations. However, the interferences were substantially reduced when the interferent concentration was reduced by a factor of 10. Nernstian response was maintained in the presence of bovine serum albumin (BSA), serum (HS) and aI acid glycoprotein (AAG). A polyurethane, (Tecoflex SG80) membrane matrix was more biocompatible when compared to PVC as evidenced by lower @ (internal) shifts with the former.