Local and systemic concentrations of tumour necrosis factor-α, interleukin-6 and interleukin-8 in bacterial osteomyelitis

✍ Scribed by Sue Fullilove; John Jellis; Scan P.F. Hughes; Daniel G. Remick; Jon S. Friedland

• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 613 KB
• Volume: 94
• Category: Article
• ISSN: 0035-9203
• DOI: 10.1016/s0035-9203(00)90284-0

✦ Synopsis

Osteomyelitis is a major cause of morbidity worldwide but there are few data investigating pathogenesis of infection and no investigations into local secretion of proinflammatory cytokines in patients. Tumour necrosis factor-a (TNF), interleukin (IL)-6 and IL-8 concentrations were measured in pus of infected bone from 30 Zambian.patients with chronic osteomyelitis (principally caused by Staphylococcus aureus), in plasma, and after lipopolysaccharide stimulation of whole-blood leucocytes. Patients had reduced body mass index compared to controls (P = 0.025) and an acute-phase response. Elevated concentrations of proinflammatory cytokines were detected in bone compared to plasma (all P < 0.0002). Bone IL-8 concentrations were greater than IL-S levels after lipopolysaccharide stimulation ofwhole blood (P < 0.0 1). In contrast, systemic and ex-vivo-stimulated concentrations of proinflammatory cytokine were similar in patients and controls, despite differences in body mass index and an acute-phase response. In summary, we observed marked local TNF, IL-6 and IL-8 secretion in established bacterial osteomyelitis without systemic cytokine release.