𝔖 Bobbio Scriptorium
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Live cell imaging of phosphoinositides with expressed inositide binding protein domains

✍ Scribed by Péter Várnai; Tamas Balla


Book ID
116986969
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
631 KB
Volume
46
Category
Article
ISSN
1046-2023

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We describe the quantitative monitoring of TATA-binding protein (TBP) localization and expression in living Saccharomyces cerevisiae cells. We replaced the endogenous TBP with a green fluorescent protein (GFP) • TBP fusion, which was imaged quantitatively by laser scanning confocal microscopy (LSCM)