Liquid ion-exchange chromatography under pressure of milk oligosaccharides using a pulsed amperometric detector

✍ Scribed by Wei Tong Wang; David Zopf

• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 869 KB
• Volume: 189
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(89)84081-9

✦ Synopsis

GlcpNAc-(1+3)-p-D-Galp-(1+4)-D-Glc]. 2'-Fucosyllactose [a-L-Fucp-(1+2)-P-~-Galp-(1+4)-D-Glc] and 3fucosyllactose {cr-D-Galp-(1+4)-[a-L-Fucp-(l-+3)@-Glc} were separated completely under a wide range of conditions. Introduction of substituents at either the O-3 or O-4 of 2-acetamido-2-deoxy-D-glucose produced large differences in retention: lacto-N-tetraose and lacto-N-neotetraose [PD-Gal-(1+4)-PD-GlcpNAc-(l-*3)-PD-Galp-(1+4)-D-Glc]

could be separated by more than 8 min. The l.c. ion-exchange chromatographic system described represents a useful addition to existing methods for separating oligosaccharides derived from glycoproteins and glycolipids.