The critical volume fractions pertaining to the formation of DNA liquid crystals were obtained from polarization microscopy, 31 P-nmr, and phase separation experiments. The DNA length (approximately one to two times the persistence length 50 nm), ionic strength, and counterion variety dependencies a
Liquid Crystal Formation in Supercoiled DNA Solutions
β Scribed by Zakharova, Svetlana S.; Jesse, Wim; Backendorf, Claude; van der Maarel, Johan R.C.
- Book ID
- 118514738
- Publisher
- Biophysical Society
- Year
- 2002
- Tongue
- English
- Weight
- 475 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0006-3495
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## Abstract This paper analyzes equilibrium superhelical cruciform formation in a topologial domain of DNA containing inverted repeat sequences. The cruciform conformation is shown to be stable when the molecule is sufficiently negatively supercoiled but not when it is positively supercoiled. For a