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Liquid Chromatography−Fourier Transform Ion Cyclotron Resonance Mass Spectrometric Characterization of Protein Kinase C Phosphorylation

✍ Scribed by Chalmers, Michael J.; Quinn, John P.; Blakney, Greg T.; Emmett, Mark R.; Mischak, Harold; Gaskell, Simon J.; Marshall, Alan G.


Book ID
127051925
Publisher
American Chemical Society
Year
2003
Tongue
English
Weight
167 KB
Volume
2
Category
Article
ISSN
1535-3893

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Several forms of diacetyl-reducing enzyme were found to exist in the human liver cytosol. Three (DAR-2, DAR-5, and DAR-7) of them were purified as a single band on SDS-PAGE by a combination of a few kinds of column chromatographies. The in-gel tryptic digests of the purified enzymes were analyzed by