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Liquid chromatography with ultraviolet absorbance–mass spectrometric detection and with nuclear magnetic resonance spectrometry: a powerful combination for the on-line structural investigation of plant metabolites

✍ Scribed by Jean-Luc Wolfender; Karine Ndjoko; Kurt Hostettmann

Book ID
108339290
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
943 KB
Volume
1000
Category
Article
ISSN
1873-3778

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The use of preparative high performance liquid chromatography with tandem mass spectrometric directed fraction collection for the isolation and characterisation of drug metabolites in urine by nuclear magnetic resonance spectroscopy and liquid chromatography/sequential mass spectrometry
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Preparative high performance liquid chromatography (HPLC) coupled to tandem mass spectrometry has been used successfully for the isolation of several drug metabolites from urine. Nuclear magnetic resonance (NMR) spectroscopy has been employed to determine the exact chemical structure of these metabo