Liquid chromatographic separations of fluorescent 1,N6-ethenoadenosine derivatives of AMP, ATP and NAD
✍ Scribed by J. Kovář; J. Turánek; H. Zábršová; V. Kahle
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 532 KB
- Volume
- 360
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
High-performance reversed-phase (C,,) and anion-exchange chromatography of the title compounds is reported. Stationary phases of both types proved to be very convenient for an efficient analysis of the preparations of these compounds. Anionexchange columns with quaternary amines (Pharmacia Mono Q columns) and those with tertiary amines (DEAE-Spheron columns) yielded comparable results. Identification of the peaks was performed by means of ultraviolet and fluorescence spectroscopic measurements. Microgram to miligram quantities of the title substances can be separated in less than 20 min using analytical columns. For preparative purposes, up to 30 mg of these compounds can be separated on larger DEAE-Spheron columns (120 x 10 mm I.D.) in a single injection. Analysis of the reaction mixtures [containing oxidized nicotinamide-adenine dinucleotide (NAD) and variable amounts of chloroacetaldehyde under different conditions] enabled us to modify the reaction and separation conditions to increase the yield of 1,N6-etheno-NAD.