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Liquid chromatographic separation and stereoselective detection of l- and d-amino acids with catalytic reaction detection using immobilized enzymes

✍ Scribed by E. Domínguez; G. Marko-Varga; M. Carlsson; L. Gorton

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 361 KB
Volume: 8
Category: Article
ISSN: 0731-7085
DOI: 10.1016/0731-7085(90)80127-b

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✦ Synopsis

A post-column LC detection system is described for the stereoselective detection of L- and D-amino acids. The effluent of the LC column passes an immobilized enzyme reactor (IMER) containing either L- or D-amino acid oxidase. The hydrogen peroxide formed in this reactor by the oxidation of the amino acids is then transported to a second IMER containing horse-radish peroxidase. With the addition of 4-aminophenazone and dichlorophenosulphonyl chloride to the carrier, the hydrogen peroxide is reacted to form a red-coloured complex which is detected in a flow-through photometric cell at 514 nm. Applications to the analysis of amino acids in bovine and human sera are described.

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