Liquid chromatographic bioanalytical determination of ropivacaine, bupivacaine and major metabolites

✍ Scribed by T. Arvidsson; Y. Askemark; M. M. Halldin

• Book ID: 101276503
• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 83 KB
• Volume: 13
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199906)13:4<286::aid-bmc862>3.0.co;2-m

✦ Synopsis

Bioanalytical methods for the determination of ropivacaine, bupivacaine and their major metabolites in urine and blood plasma are presented. Ropivacaine is a new local anaesthetic drug mainly used for surgery and for postoperative pain relief. The samples are hydrolysed and cleaned using solid-phase extraction and analysed using ion-pair reversed-phase liquid chromatography with gradient elution. The analytes are detected using UV at 210 nm. The methods are highly selective and the limits of quantification were 1 mM in urine and 0.1 mM in plasma, respectively. The between-day variance was generally below 3% (RSD).