Liquid chromatographic and spectrofluorimetric determination of aspartame and glutamate in foodstuffs following fluorescamine fluorigenic labelling
✍ Scribed by F. García Sánchez; A. Aguilar Gallardo
- Book ID
- 102983623
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 881 KB
- Volume
- 270
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
A spectrofluorimetric method for determining aspartame (AS) and sodium glutamate (GL) based on labelling with fluorescamine (FC) was developed. Mixtures of AS and GL were analysed by isodifferential synchronous derivative spectrofluorimetry. R.S.D.s of 6.4% and 3.0% were obtained by the individual methods. Detection limits of 0.03 and 0.1 p.g ml I were obtained for AS and GL, respectively. On the basis of the FC derivatization procedure, a liquid chromatographic method to determine AS and GL was developed. R.S.D.s of 0.62 and 0.34% were obtained with fluorimetric detection and 2.26 and 3.14% with photometric detection at 395 nm. Detection limits were 0.04 and 0.02 /xg ml-X (spectrofluorimetric detection) and 0.2 and 0.1 /xg ml 1 (spectrophotometric detection) for AS and GL, respectively. These methods were applied to the determination of AS in carbonated soft drinks and GL analyses in commercial dried soups.