Liposomes with a large trapping capacity prepared by freezing and thawing of sonicated phospholipid mixtures

Reconstitution of sodium channels in lar

Reconstitution of sodium channels in large liposomes formed by the addition of acidic phospholipids and freeze-thaw sonication

✍ Vanessa Miguel; Domingo Balbi; Cecilia Castillo; Raimundo Villegas 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 822 KB

Phosphatidylcholine (PC) alone or with phosphatidylethanolamine (PE) are sufficient for the reconstitution of Na+ channels in planar lipid bilayers. However, when Na+ channels were first reconstituted into liposomes using the freeze-thaw-sonication method, addition of acidic phospholipids, such as p