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Liposomes as tools to study drug diffusion and toxin-induced leaks

✍ Scribed by Florence Levillain; Gilbert Lanéelle

Publisher
The American Society for Biochemistry and Molecular Biology
Year
2002
Tongue
English
Weight
64 KB
Volume
30
Category
Article
ISSN
1470-8175

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✦ Synopsis

Abstract

Multilayered vesicles made of egg‐phosphatidylcholine and of phosphatidic acid were used to teach in a 4‐h session of practical work with a low cost spectrophotometer how to determine osmolarity inside multilayered vesicles and to show, by using two anti‐tuberculous drugs (isonicotinic acid hydrazide, p‐aminosalicylate), that a small and non‐ionized molecule diffused freely through phospholipid vesicles, whereas a charged one did not. In addition, the permeabilizing effect of melittin, a membrane‐targeted bee‐venom toxin, was tested.

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