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Liposome-mediated mycelial transformation of filamentous fungi

โœ Scribed by Chai, Ran; Zhang, Guang; Sun, Qiang; Zhang, Mingyue; Zhao, Shuaiju; Qiu, Liyou


Book ID
121233204
Publisher
Elsevier
Year
2013
Tongue
English
Weight
679 KB
Volume
117
Category
Article
ISSN
1878-6146

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Transformation of Neurospora crassa spheroplasts is reported for three different genes, using uncloned Neurospora DNA, both naked and encapsulated in synthetic phosphatidylserine liposomes. Whereas transformation by naked DNA is DNase-sensitive, that by liposomes is not. Per unit of transforming DNA