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Liposome Destabilization by a 2,7-Diazapyrenium Derivative Through Formation of Transient Pores in the Lipid Bilayer

✍ Scribed by Romina Zappacosta; Monica Semeraro; Massimo Baroncini; Serena Silvi; Massimiliano Aschi; Alberto Credi; Antonella Fontana


Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
335 KB
Volume
6
Category
Article
ISSN
1613-6810

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✦ Synopsis


Abstract

The effect of the luminescent heteroaromatic electron acceptor N,N′‐dimethyl‐2,7‐diazapyrenium dichloride (DM‐DAP^2+^) on the stability of 1‐palmitoyl‐2‐oleoylphosphatydilcholine (POPC) liposomes is determined on the basis of the rate of release of different fluorescent probes entrapped within the liposome. The experiments show that DM‐DAP^2+^ exerts a substantial destabilizing action on the liposomal bilayer, particularly at low concentrations. Molecular dynamics simulations suggest that the activity of DM‐DAP^2+^ is related to its tendency to surround itself with water molecules, conceivably favoring the formation of transient pores across the bilayer.