Lipophilic arsenic compound(s) in the liver of a tiger shark (Galeocerdo cuvier)

✍ Scribed by Mariko Miyajima; Noriaki Hamada; Etsuro Yoshimura; Akira Okubo; Sunao Yamazaki; Shozo Toda

• Book ID: 101571576
• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 410 KB
• Volume: 2
• Category: Article
• ISSN: 0268-2605
• DOI: 10.1002/aoc.590020416

✦ Synopsis

Homogenized aliquots (100 g) of the liver (8.4 kg, 5 pg As g-') of a tiger shark (Guleocerdo cuvier) were extracted with chloroform/methanol, and the extracts purified by countercurrent extraction (hexane/87% ethanol), silica gel column chromatography (chloroform/methanol mixtures as mobile phases), and silica gel thin-layer chromatography (chloroformlmethanoliacetic acid). The purified samples (24 mg arsenic g-') gave no "P NMR signal, but gave 'H and I3C NMR signals with similarities to those of dipalmitoylphosphatidic acid and salad oil and also signals indicative of the presence of methylated arsenic compounds. The sample could contain a diacyl glyceride with a methylated arsenic group.