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Lipolytic enzyme production in batch and fed-batch cultures of Ophiostoma piceae and Fusarium oxysporum

✍ Scribed by Candan Tamerler; Tajalli Keshavarz

Publisher: Wiley (John Wiley & Sons)
Year: 2000
Tongue: English
Weight: 128 KB
Volume: 75
Category: Article
ISSN: 0268-2575
DOI: 10.1002/1097-4660(200009)75:9<785::aid-jctb293>3.0.co;2-r

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✦ Synopsis

Lipase and esterase production by Ophiostoma piceae and Fusarium oxysporum were enhanced and extended by developing a fed-batch process in stirred tank reactors. Fed-batch strategy improved lipolytic enzyme production from Ophiostoma piceae in both 2 and 20 dm 3 stirred tank reactors. However, fed-batch fermentation of Fusarium oxysporum in the 2 dm 3 reactor was more effective than both batch and fed-batch fermentations in the 20 dm 3 reactor. When a medium composed of only carbon and nitrogen source was intermittently fed to the cultures, the maximum speci®c lipase activity was improved by more than 80% and 35% in Ophiostoma piceae and Fusarium oxysporum cultures respectively. The maximum speci®c esterase activity was improved by 20% and 15% in Ophiostoma piceae and Fusarium oxysporum cultures respectively. The duration of production for both fungi extended from 144 to 216 h compared with a batch culture under the same condition.

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