Lipochito-oligosaccharides re-initiate root hair tip growth in Vicia sativa with high calcium and spectrin-like antigen at the tip
✍ Scribed by Norbert C. A. D; Ruijter; Martin B. Rook; Ton Bisseling; Anne Mie C. Emons
- Book ID
- 104463724
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 437 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0960-7412
No coin nor oath required. For personal study only.
✦ Synopsis
Summary
Lipochito‐oligosaccharides, Nod factors secreted by Rhizobium bacteria, are signal molecules that induce deformation of root hairs of their host plant. A bioassay was used for deformation, and the cytological changes induced by specific lipochito‐oligosaccharides in root hairs of Vicia sativa L. (vetch), grown between glass slides, were examined. In the assay, root hairs of a particular developmental stage, those that were terminating growth, were susceptible to deformation. These hairs obtained characteristics of tip‐growing cells again: (i) a polar cytoplasmic organization and reverse fountain streaming, (ii) an accumulation of a spectrin‐like antigen at the tip, and (iii) a tip‐focused calcium gradient. Calcium gradients were visualized in Indo‐1 loaded root hairs with UV confocal microscopy and ratio‐imaging. The results show that hairs respond to the bacterial signal by recovering cytoplasmic polarity and exocytosis.