Lipid-Protein Interactions: Methods and Protocols

✍ Scribed by Jörg H. Kleinschmidt

• Publisher: Springer New York; Humana
• Year: 2019
• Tongue: English
• Leaves: 685
• Series: Methods in Molecular Biology 2003
• Edition: 2nd ed.
• Category: Library

✦ Synopsis

This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications. Additional protocols were added to examine lipid-protein interactions by mass spectrometry, to use protein microarrays to investigate large sets of various proteins, to study membrane protein dynamics by UV resonance Raman spectroscopy, to analyze peptide-induced pore formation in membranes, and to investigate folding and insertion of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Lipid-Protein Interactions: Methods and Protocols, Second Edition is an essential resource for all researchers who are interested in obtaining up-to-date and comprehensive information about membrane structure and function.

✦ Table of Contents

Front Matter ....Pages i-xiv
Multiscale Modeling and Simulation Approaches to Lipid–Protein Interactions (Roland G. Huber, Timothy S. Carpenter, Namita Dube, Daniel A. Holdbrook, Helgi I. Ingólfsson, William A. Irvine et al.)....Pages 1-30
Quartz Crystal Microbalances as Tools for Probing Protein–Membrane Interactions (Søren B. Nielsen, Daniel E. Otzen)....Pages 31-52
Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid Membranes (Aleksandra Šakanovič, Vesna Hodnik, Gregor Anderluh)....Pages 53-70
Thermodynamic Analysis of Protein–Lipid Interactions by Isothermal Titration Calorimetry (Musti J. Swamy, Rajeshwer S. Sankhala, Bhanu Pratap Singh)....Pages 71-89
Differential Scanning Calorimetry of Protein–Lipid Interactions (Olga Cañadas, Cristina Casals)....Pages 91-106
Imaging and Force Spectroscopy of Single Transmembrane Proteins with the Atomic Force Microscope (K. Tanuj Sapra)....Pages 107-144
Kinetics of Insertion and Folding of Outer Membrane Proteins by Gel Electrophoresis (Andre Schüßler, Sascha Herwig, Jörg H. Kleinschmidt)....Pages 145-162
Optimized Negative-Staining Protocol for Lipid–Protein Interactions Investigated by Electron Microscopy (Jianfang Liu, Hao Wu, Changyu Huang, Dongsheng Lei, Meng Zhang, Wei Xie et al.)....Pages 163-173
Probing Heterogeneous Lipid Interactions with Membrane Proteins Using Mass Spectrometry (John W. Patrick, Arthur Laganowsky)....Pages 175-190
Protein Microarrays and Liposome: A Method for Studying Lipid–Protein Interactions (Samuel Herianto, Chien-Sheng Chen, Heng Zhu)....Pages 191-199
Structural Investigations of Protein–Lipid Complexes Using Neutron Scattering (Luke A. Clifton, Stephen C. L. Hall, Najet Mahmoudi, Timothy J. Knowles, Frank Heinrich, Jeremy H. Lakey)....Pages 201-251
Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid Environment (Koichi Matsuo, Kunihiko Gekko)....Pages 253-279
FTIR Analysis of Proteins and Protein–Membrane Interactions (Suren A. Tatulian)....Pages 281-325
UV Resonance Raman Spectroscopy as a Tool to Probe Membrane Protein Structure and Dynamics (DeeAnn K. Asamoto, Judy E. Kim)....Pages 327-349
Analyzing Transmembrane Protein and Hydrophobic Helix Topography by Dual Fluorescence Quenching (Gregory A. Caputo, Erwin London)....Pages 351-368
Förster Resonance Energy Transfer as a Tool for Quantification of Protein–Lipid Selectivity (Luís M. S. Loura, Manuel Prieto, Fábio Fernandes)....Pages 369-382
A Guide to Tracking Single Membrane Proteins and Their Interactions in Supported Lipid Bilayers (Evan L. Taylor, Kumud Raj Poudel, James A. Brozik)....Pages 383-414
Fluorescence Correlation Spectroscopy to Examine Protein–Lipid Interactions in Membranes (Viktoria Betaneli, Jonas Mücksch, Petra Schwille)....Pages 415-447
Membrane Pore Formation by Peptides Studied by Fluorescence Techniques (Suren A. Tatulian, Nabin Kandel)....Pages 449-464
Folding of β-Barrel Membrane Proteins into Lipid Membranes by Site-Directed Fluorescence Spectroscopy (Lisa Gerlach, Omkolsum Gholami, Nicole Schürmann, Jörg H. Kleinschmidt)....Pages 465-492
EPR Techniques to Probe Insertion and Conformation of Spin-Labeled Proteins in Lipid Bilayers (Enrica Bordignon, Svetlana Kucher, Yevhen Polyhach)....Pages 493-528
Studying Lipid–Protein Interactions with Electron Paramagnetic Resonance Spectroscopy of Spin-Labeled Lipids (Tibor Páli, Zoltán Kóta)....Pages 529-561
Solid-State NMR Approaches to Study Protein Structure and Protein–Lipid Interactions (Christopher Aisenbrey, Evgeniy S. Salnikov, Jesus Raya, Matthias Michalek, Burkhard Bechinger)....Pages 563-598
Solution NMR Spectroscopy for the Determination of Structures of Membrane Proteins in a Lipid Environment (Ashish Arora)....Pages 599-643
Nanodiscs as a New Tool to Examine Lipid–Protein Interactions (Ilia G. Denisov, Mary A. Schuler, Stephen G. Sligar)....Pages 645-671
Back Matter ....Pages 673-682

✦ Subjects

Life Sciences; Protein Science; Lipidology; Mass Spectrometry