Lipid-protein interactions in membranes: Effect of lipid composition on mobility of spin-labeled cysteine residues in yeast plasma membrane
✍ Scribed by Esfahani, Mojtaba ;Solomon, Daniel J. ;Mele, Lisa ;Teter, M. Noel
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1979
- Tongue
- English
- Weight
- 515 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0091-7419
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✦ Synopsis
In order to gain direct evidence for lipid-dependent protein conformation in membrane, effects of modification of lipid composition on mobility of spinlabeled cysteine residues were investigated in the plasma membrane of the yeast Saccharomyces cerevisiae. Conversion of the bulk of phospholipids t o diglycerides by treatment of the membrane with phospholipase C substantially enhanced spectral anisotropy. However, alteration of the viscosity of the lipidbilayer by enriching the membrane with palmitelaidic or oleic acid had no effect on mobility of spin-labeled cysteine residues. These observations indicate that while the spin-labeled residues are not in direct contact with the lipid core of the membrane, there are lipid-protein interactions t o the extent that removal of the polar portion of the bulk of phospholipids induces conformational changes in proteins, which in turn restrict mobility of these residues. It is concluded that conformation of membrane proteins depends o n lipid structure and that phospholipids have a role in preserving the native conformation of proteins.