Lipid mobility in Acholeplasma membranes using deuteron magnetic resonance

✍ Scribed by E. Oldfield; D. Chapman; W. Derbyshire

• Book ID: 103039994
• Publisher: Elsevier Science
• Year: 1972
• Tongue: English
• Weight: 613 KB
• Volume: 9
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(72)90034-5

✦ Synopsis

Acholeplasma laidlawii B have been grown on media supplemented with perdeuterated lauric and palmitic acids. The lauric acid was shown to be elongated to myristic acid-d23 and palmitic acid-dza, and these acids were incorporated into the membrane lipids.

Infrared measurements indicated the membrane proteins were predominantly ct-helical. Deuteron magnetic resonance spectroscopy of the deuterated lipids in intact membranes indicated that the lipids were in a state of mobility rather similar to that of the gel state of dimyristoyl lecithin. This is consistent with our interpretation of results obtained by differential scanning calorimetry and X-ray diffraction. The existence of rigid lipid regions in organisms lacking cholesterol may not be uncommon, and may occur in E. coli also.