Lipid characterization in an animal model of atherosclerosis using NMR spectroscopy and imaging

✍ Scribed by R. F. G. Booth; A. C. Honey; J. F. Martin; J. C. Lindon; R. D. Farrant; T. A. Carpenter; L. D. Hall

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 705 KB
• Volume: 3
• Category: Article
• ISSN: 0952-3480
• DOI: 10.1002/nbm.1940030209

✦ Synopsis

High resolution ' H and =C NMR spectroscopic measnrements including 'H/"C 2D correlation and magnetic resoDpnee imaging 0 studies, have been carried ont on intact rabbit aortic tissues CT uluo using animals fed both normal a d high cholesterol diets. me tcsolQ sbow that ' H and "C NMR speetroseopy can distinguish mobile lipids and can differentiate between n o d triglyceride content and cholesterol-enriched lipids, in intact tissue. There were considerable dirmerences in the level of deposition of rbdesteryl esters m animals all fed on the same diet. Contimalion is presented of temperature-dependent dilFerences in mobility and organization between the triglycerides fonnd m control tissue and the cholesteryl esters found in aortas from high lipid diet animals. Water-slrppressed MRI showed evidence of lipid accumnlation in the aortas of high cholesterol diet rabbits. It is concloded that the hypercholesterolaemic rabbit model of atherosclerosis, coupled with saeb NMR methods, may d e r a noniovasive mcthod of monitoring disease development, allowing the evaluation of the effect of therapentic agents on the progress of atherosclerosis. 0 Heyden & Son Limited, 1990 ccc-0952-3480/90/oos5-0100 so3.00