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Lipase-catalyzed acidolysis of menhaden oil with conjugated linoleic acid: Effect of water content

✍ Scribed by Carlos F. Torres; Charles G. Hill Jr.

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
144 KB
Volume
78
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

The effect of the water content on the lipase‐catalyzed (Candida rugosa) interesterification (acidolysis) of menhaden oil with conjugated linoleic acid was studied for amounts of added water ranging from 0–4% (w/w). The rate of the acidolysis reaction increased with increasing water content, but the corresponding percentage of n‐3 fatty acids liberated also increased. The implications of water content for minimization of the release of n‐3 fatty acid residues while maximizing incorporation of CLA are discussed. Β© 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 509–516, 2002.

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## Abstract Acidolysis of butteroil with free conjugated linoleic acid (CLA) was studied in a packed bed reactor containing an immobilized __Candida antarctica__ (fraction B) lipase. Kinetic data were used to develop quantitative reversible rate expressions of the general Michaelis–Menten form that