Linking Mass Spectrometry and Slab-Polyacrylamide Gel Electrophoresis by Passive Elution of Lipopolysaccharides from Reverse-Stained Gels: Analysis of Gel-Purified Lipopolysaccharides from Haemophilus i nfluenzae Strain Rd

Matrix-assisted laser desorption/ionizat

Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry of lipopolysaccharide species separated by slab-polyacrylamide gel electrophoresis: High-resolution separation and molecular weight determination of lipooligosaccharides from Vibrio fischeri strain HMK

✍ Elder Pupo; Nancy J. Phillips; Bradford W. Gibson; Michael A. Apicella; Eugenio 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 128 KB 👁 1 views

We recently demonstrated that the combined use of lipopolysaccharide (LPS) reverse staining and high-efficiency passive elution techniques can be successfully used as a suitable interface between LPS slab-gel separation and electrospray ionization-mass spectrometry (ESI-MS) of LPS-derived oligosacch