𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Linker mutagenesis in the lacZ gene of Escherichia coli yields variants of active β-galactosidase

✍ Scribed by Astrid BREUL; Wolfgang KUCHINKE; Brigitte von WILCKEN-BERGMANN; Benno MÜLLER-HILL


Book ID
115128344
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
409 KB
Volume
195
Category
Article
ISSN
1432-1327

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Classification and intragenic position o
✍ Langridge, J. ;Campbell, John H. 📂 Article 📅 1969 🏛 Springer 🌐 English ⚖ 618 KB

Following treatment with N-methyl-N'-nitro-N-nitrosoguanidine, 1,257 mutants of Escherichia coli K]2 were isolated on lactose-tetrazolium medium. Of these mutants, 345 were lactose-negative and lacked appreciable fi-galactosidase activity. About half of these enzyme-deficient mutants had lost the wh