Linkage of phosphoribosylpyrophosphate synthetases 1 and 2,Prps1andPrps2, on the mouse X Chromosome

✍ Scribed by K. Lee; B. Keitz; M. Taira; V. M. Chapman

• Publisher: Springer-Verlag
• Year: 1994
• Tongue: English
• Weight: 466 KB
• Volume: 5
• Category: Article
• ISSN: 0938-8990
• DOI: 10.1007/bf00411455

✦ Synopsis

The X Chromosome (Chr) genes for phosphoribosylpyrophosphate synthetases 1 and 2, Prpsl and Prps2, were mapped on the mouse X Chr with interspecific backcrosses between C57BL/6 (B6) and M. spretus (S). Southern analysis showed that Prpsl mapped between Plp and DXWas31, a mouse X Chr region that is homologous to Xq21-24 on the human X Chr while Prps2 mapped between DXWas31 and Amg, a region that is homologous to the map position of PRPS2 on Xp22 of the human X Chr. Additionally, other restriction fragments highlighted by PRS II showed autosomal segregation. In situ hybridization and FISH analysis of metaphase chromosome spreads prepared from lymphocytes of B6 or S male mice confirmed that there were in fact two different locations on the X Chr, X F1-2 and X F2-3 for Prpsl and 2 respectively, as well as two autosomal sites for Prps-like genes.