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Linkage determination of alditol disaccharides using high-energy collision-induced dissociation spectra of sodium cationized molecules

✍ Scribed by Vladimír Kováčik; Ján Hirsch; Wigger Heerma


Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
118 KB
Volume
11
Category
Article
ISSN
0951-4198

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✦ Synopsis


Oligosaccharide alditols containing a deuterium atom at the alditol C-1 position can be used to obtain information about the masses and linkages of the various residues. The high-energy collision-induced dissociation (CID) spectra of the protonated compounds show mainly glycosidic bond cleavage with charge retention on the alditol containing Y-type ions. The CID spectra of the sodium-cationized compounds show additional fragment ions resulting from cross-ring cleavages. Moreover the elimination of methanol, formaldehyde and ethylene glycol molecules, as deuterium labelled or unlabelled species, appeared to provide a reliable criterion to determine the linkage positions at the alditol unit.