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Linewidth-Resolved 15N HSQC, a Simple 3D Method to Measure 15N Relaxation Times from T1 and T2 Linewidths

✍ Scribed by Sami Heikkinen; Ilkka Kilpeläinen

Publisher: Elsevier Science
Year: 2001
Tongue: English
Weight: 90 KB
Volume: 151
Category: Article
ISSN: 1090-7807
DOI: 10.1006/jmre.2001.2383

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✦ Synopsis

A three-dimensional approach for measuring 15 N relaxation times is described. Instead of selecting particular values for the relaxation period, in the proposed method the relaxation period is incremented periodically in order to create a 3D spectrum. This additional frequency domain of the transformed spectrum contains the relaxation time information in the T 1 and T 2 linewidths, and thus the longitudinal and transverse 15 N relaxation times can be measured without determination of 2D cross peak volumes/intensities and subsequent curve fitting procedures.

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Methods for the Measurement of 1JNCα and

Methods for the Measurement of 1JNCα and 2JNCα from a Simplified 2D 13Cα-Coupled 15N SE–HSQC Spectrum

✍ Sami Heikkinen; Perttu Permi; Ilkka Kilpeläinen 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 119 KB

Two methods for the measurement of (2)J(NCalpha) and (1)J(NCalpha) in (15)N/(13)C-labeled small and medium-size proteins are described. The current approach is based on simplified (13)C(alpha)-coupled (15)N HSQC spectra, where the two (2)J(NCalpha) doublets are separated into two subspectra correspo