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Linearly polarized second harmonic generation microscopy reveals chirality

✍ Scribed by Valev, V. K.; Silhanek, A. V.; Smisdom, N.; De Clercq, B.; Gillijns, W.; Aktsipetrov, O. A.; Ameloot, M.; Moshchalkov, V. V.; Verbiest, T.

Book ID
115413221
Publisher
Optical Society of America
Year
2010
Tongue
English
Weight
664 KB
Volume
18
Category
Article
ISSN
1094-4087

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We describe the principles and characteristics of second-harmonic generation imaging (SHGI) and explore various methods for optimization of the technique. Second-harmonic imaging is optimized for ultrashort laser pulses, high numerical aperture microscope objectives, a highly sensitive non-descanned