Linear and Quadratic Relationships between Retention and Organic Modifier Content in Eluent in Reversed Phase High-Performance Liquid Chromatography: A Systematic Comparative Statistical Study
✍ Scribed by Ba¸czek, Tomasz ;Markuszewski, Michal ;Kaliszan, Roman ;van Straten, Marion A. ;Claessens, Henk A.
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 90 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Statistical evaluation of linear and quadratic models of chromatographic retention was performed. The relationships describing retention were derived for a set of 23 carefully selected test analytes chromatographed on 18 HPLC columns using methanol-water or acetonitrile-water solutions as mobile phase. It was ascertained whether the square term in the quadratic model improves the description of chromatographic retention in a statistically significant manner. It was also checked whether the retention data extrapolated to a hypothetical neat water eluent (log k w ) obtained with the two models and the two organic modifiers are equivalent or should be considered different. The research proved that both models yield similar results and the extrapolated log k w values do not differ statistically significantly in the case of methanol-containing mobile phases. In the case of acetonitrile-water systems the log k w values obtained with linear and quadratic models are normally statistically different. Correlation analysis for log k w vs. log P parameters was performed for data determined on six columns. The best correlations were achieved employing log k w data derived with the linear model for methanol/ water systems.