Limits on modification of epitrochoid used in rotary piston machines and the effects of modification on geometric volume displacement and ripple

Selective modification of surface-expose

Selective modification of surface-exposed thiol groups in Trigonopsis variabilisD-amino acid oxidase using poly(ethylene glycol) maleimide and its effect on activity and stability of the enzyme

✍ Anita Slavica; Iskandar Dib; Bernd Nidetzky 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 253 KB 👁 2 views

## Abstract Covalent modification of purified __Trigonopsis variabilis__ D‐amino acid oxidase using maleimide‐activated poly(ethylene glycol) 5000 yielded a stable bioconjugate in which three surface‐exposed cysteine side chains were selectively derivatized. Compared with the native enzyme, the PEG