Limiting factors involved in CO2 production by starved and X-irradiated starved yeast cells

✍ Scribed by Spoerl, Edward ;Doyle, R. J. ;Thompson, M. E.

• Book ID: 102309890
• Publisher: Wiley (John Wiley & Sons)
• Year: 1965
• Tongue: English
• Weight: 489 KB
• Volume: 65
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030650215

✦ Synopsis

Studies of reduced COZ production by starved yeast cells were carried out to localize the site limiting this process and responsible for a greater production by irradiated cells. Cell-free extracts prepared before and after starvation of cells, and from irradiated and unirradiated cells, showed similar hexokinase activity and produced similar amounts of COz. These results demonstrated that rate limiting glycolytic enzymes did not decay during starvation, were not induced during a lag period i n COz production which could be overcome by glucose incubation, and were not responsible for differences in COz production between irradiated and unirradiated cells. Possible limiting factors involved in these differences include glucose transport as a consequence of differential decay during starvation, restricted cofactor synthesis and a n enzymic binding or compartmentalization.