Limitations on the stability of benzoylated DEAE-cellulose

✍ Scribed by Michelle Haber; Peter H.T. Huang; Bernard W. Stewart

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 287 KB
• Volume: 139
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90018-6

✦ Synopsis

As used for structural analysis of DNA, benzoylated DEAE-cellulose is subject to deterioration which affects chromatographic performance. This change, which specifically occurs during storage of the resin, has been characterized in terms of anomalous patterns of DNA binding. Subjecting benzoylated DEAE-cellulose to boiling in aqueous suspension, and appropriate storage, ensures maintenance of normal affinity for DNA.