Limitations of the graphical analysis of elution curves

✍ Scribed by F. Hagemeijer; P. van Remoortere

• Book ID: 104155155
• Publisher: Elsevier Science
• Year: 1969
• Tongue: English
• Weight: 740 KB
• Volume: 25
• Category: Article
• ISSN: 0022-5193
• DOI: 10.1016/s0022-5193(69)80062-7

✦ Synopsis

With the use of an analogue computer, elution curves have been simulated from theoretical mathematical models. These curves are submitted to the graphical analysis currently used. Differences between theoretical and measured data correspond essentially to the fact that back-diffusion is overlooked. This can be corrected with Huxley's equations whenever only two compartments actually exchange with the washout solution. When more than two compartments can be individualized, the measured contents of these compartments are good approximations of the actual contents whenever the time constants of exchange are very different. On the other hand, the measured time constants are only of the same order of magnitude as the actual time constants and result from the interrelations between outflux and back-diffusion.

The analogue computer should be used to simulate elution curves in order to verify the mathematical model for biological preparations in washout experiments.