Limitations of HPLC for the detection of β-exotoxin in culture filtrates ofBacillus thuringiensis

✍ Scribed by Donovan E. Johnson; Robert E. Peterson

• Book ID: 104789781
• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 324 KB
• Volume: 17
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00510421

✦ Synopsis

High performance liquid chromatography (HPLC) was used to detect and quantify fl-exotoxin, a phosphorylated adenine nucleotide derivative produced as an excreted metabolite by several strains of Bacillus thuringiensis. The assay was rapid and quantitative for purified fl-exotoxin standards. However, peak height failed to correlate fl-exotoxin concentration in crude culture filtrates with biological activity toward house fly larvae. Unrelated compounds (from non-fl-exotoxin producers) co-eluted with fl-exotoxin, thereby making the technique an unreliable method for toxin detection and quantification.