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Limitation of macrophage production in long-term marrow cultures containing prostaglandin E

โœ Scribed by N. Williams; H. Jackson

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
612 KB
Volume
103
Category
Article
ISSN
0021-9541

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โœฆ Synopsis

Addition of prostaglandin Ez (PGE,) significantly altered the cellular composition of murine long-term bone marrow cultures. After 4-5 weeks of culture, increased cellularity in the suspension phase was observed in all cultures containing prostaglandin. These suspension cells contained markedly higher proportions of differentiated neutrophils than did cells cultured in the absence of PGE,. Granulocyte-macrophage progenitor cell levels in the suspension layer were increased 3-20 fold after five weeks in prostaglandin-containing cultures compared with control cultures. Fewer cells comprised the adherent layer in cultures containing prostaglandin. The number of macrophages in this layer was reduced 3-8 fold in these cultures compared with control cultures, while the number of granulocytes was increased 2-3 fold. The progenitor cells biased toward macrophage development were selectively inhibited in the cultures with PGE,. There was no significant effect of PGE, on pluripotent stem cell

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