Limbal cell autograft transplantation for severe ocular surface disorders

Optimization of culture conditions for a

Optimization of culture conditions for an efficient xeno-feeder free limbal cell culture system towards ocular surface regeneration

✍ Viji Mary Varghese; Tilak Prasad; T.V. Kumary 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 568 KB

## Abstract Ex vivo expansion of limbal stem cells from a small biopsy and its subsequent transplantation is the golden choice of treatment for limbal stem cell deficiency. Use of murine 3T3 feeder layer is a prerequisite for this ex vivo expansion. There is an ever‐increasing demand for feeder fre