Light scattering and hydrodynamic properties of linear and circular bacteriophage lambda DNA

✍ Scribed by J. R. Dawson; J. A. Harpst

• Publisher: Wiley (John Wiley & Sons)
• Year: 1971
• Tongue: English
• Weight: 615 KB
• Volume: 10
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.360101211

✦ Synopsis

Low-angle light scattering, sedimentation velocit,y, and intrinsic viscosity measurements have been made on circular and linear forms of lambda (A) bacteriophage DNA. Available equations, used to relate hydrodynamic parameters of both forms to the moleciilar weight, give relatively consistent values of particle weights which essentially agree with the light-scattering resii1t.s.

3. X lo6 for X DNA was obtained in good agreement with literature values of (31-33) X lo6. The linear X DNA has a larger root-mean-square radius than the circular molecule, when determined by light scattering, but the difference does riot appear to be as large as expected from hydrodynamic data. The two forms also show significantly different angular dishibntions of scattered light intensities which agree only qualitatively with those derived from existing theory. The light-scattering results suggest that further experiments and modifications of available theories shonld be undertaken.

An average molecular weight of (34