Light-induced fluorescence spectroscopy of adenomas, adenocarcinomas and non-neoplastic mucosa in human colon I. In vitro measurements
✍ Scribed by Renato Marchesini; Marco Brambilla; Emanuele Pignoli; Giovanni Bottiroli; Anna Cleta Croce; Marco Dal Fante; Pasquale Spinelli; Silvana di Palma
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 810 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1011-1344
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✦ Synopsis
In an attempt to evaluate whether induced fluorescence could be exploited to discriminate neoplastic from non-neoplastic tissue, fluorescence spectroscopy was performed at 450-800 nm on 83 biopsy specimens of colonic mucosa. Measurements showed that fluorescence spectra of adenoma, adenocarcinoma and non-neoplastic mucosa manifest dissimilar patterns. Nine variables, whose photophysical and/or biological bases need further investigation, were derived from the spectra. Discriminant functions between the groups of lesions were determined by using a stepwise discriminant analysis. The diagnostic test had a sensitivity of 80.6% and 88.2%, and a specificity of 90.5% and 95.2% in discriminating neoplastic from non-neoplastic mucosa and adenoma from non-neoplastic mucosa respectively. These results suggest that fluorescence spectroscopy has the potential to improve endoscopic diagnosis of premalignant and malignant lesions of colonic mucosa.