✦ LIBER ✦

Light-induced banding (LIB) in metaphase chromosomes of Culiseta longiareolata (Diptera: Culicidae)

✍ Scribed by R. Mezzanotte; L. Ferrucci; A. Marchi

Publisher: Springer Netherlands
Year: 1980
Tongue: English
Weight: 814 KB
Volume: 51
Category: Article
ISSN: 0016-6707
DOI: 10.1007/bf00133515

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✦ Synopsis

A technique already used on Drosophila polytene chromosomes was employed on metaphase chromosomes of Culiseta Iongiareolata (Diptera: Culicidae). The coincidence between Q-bands and green fluorescence was pointed out. The centromere regions of all chromosome pairs and one telomere of the Y, although demonstrating uniformly stained C-bands, were shown to differentially stain with Coriphosphine-0 after light treatment. A base destruction mechanism, similar to that proposed for the methylene blue-light-chrtimosomal DNA interaction, has been hypothesized for explaining these findings.

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The metaphase banding pattern produced i

The metaphase banding pattern produced in the chromosomes ofCuliseta longiareolata(Diptera: Culicidae) by AluI restriction endonuclease or actinomycin-D treatment followed by acridine-orange staining

✍ R. Mezzanotte; L. Ferrucci 📂 Article 📅 1983 🏛 Springer Netherlands 🌐 English ⚖ 592 KB

Cytological preparations of Culiseta Iongiareolata were treated with restriction endonuclease AluI or actinomycin-D and then stained with acridine orange. The longitudinal differentiation obtained was related to previous cytological data found in this species and interpreted as a possible consequenc